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Fig. 6. Expression of Tgfß ligands and receptors in the mouse
foregut and early lung. (A-H) WMISH of Tgfbr1 and
Tgfbr2 showing that both receptors are diffusely expressed in the
E8.5 foregut (A,E) and in control cultured foregut at 24 hours (B,F). By 72
hours in culture, strong Tgfbr1 (C) and Tgfbr2 (G)
expression is detected in the endoderm and mesoderm of the lung region (boxed
areas in C and G, magnified in L). At E12.5, both receptors are highly
expressed in the distal lung in vivo (arrows). (I,J) WMISH of
Fgf10 (I) and X-Gal staining of Fgf10lacZ (J) expression in
E9.5 embryos reveal Fgf10 expression in the lung region (circled).
(K) X-Gal staining of an Fgf10lacZ foregut explant at 72 hours
showing strong lacZ expression in the mesenchyme associated with the
distal lung buds. (L) Boxed areas from C, G and K, showing overlap of
Fgf10lacZ, Tgfbr1 and Tgfbr2 expression in the mesenchyme at the lung
field. (M) Histological section of WMISH specimens from L showing
localization (arrowheads) of Tgfbr1 and Fgf10lacZ
expression. (N-V) WMISH of Tgfb1-3. Freshly isolated E8.5
explants (N,Q,T) and control 24-hour cultures (O,R,U) show diffuse expression
of these ligands in the foregut region, mostly in the mesoderm; signals are in
some cases associated with cardiac and vascular structures (Tgfb1,
arrowheads in N,O; Tgfb2, arrowhead in R) or endodermal structures
(Tgfb2 in S), as previously reported. In the E12.5 lung,
Tgfb1 is expressed in the subepithelial mesenchyme (P, arrowhead),
Tgfb2 is mostly restricted to the distal epithelium (S, arrowhead),
and Tgfb3 is present in mesothelial cells of the pleura and distal
epithelium and mesenchyme (V, arrowhead). en, endoderm; me, mesoderm; Fg,
foregut; Lu, lung; Ht, heart; St, stomach.
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