|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Wg is not sufficient to activate vg in ap0
wing discs. (A-D) Wild-type Drosophila discs. Vg protein
(green in A) is expressed in D-V `border' cells (yellow in A owing to
co-expression with Wg, red) as are BE reporter genes
(BE-lacZ, blue in C; BE-vgGFP, green in D). Vg is
also expressed in surrounding cells of the `pouch' and wing primordium, as are
QE reporter genes (5XQE-DsRed, red in B,C;
1XQE-lacZ, blue in D). Wg (red in A,D, green in B) is expressed in
border cells within the pouch, and in an inner and outer ring (IR, yellow
arrowhead; OR, white arrowhead) in the surrounding hinge primordium.
rn-lacZ (blue in B) is expressed throughout the pouch and in the
surrounding `rn-only' territory extending up to the inner ring of Wg.
(E-H) ap0 discs. Neither Vg (green in E, red in H),
nor BE-vgGFP (green in H), nor QE reporters (blue
in E,H, red in F,G) are expressed, and the pouch is either absent or present
only as a small cluster of rn-only cells (blue in F) surrounded by
the inner ring of Wg (red in E, green in F). Uniform ectopic expression of
UAS-Nrt-wg under C765-Gal4 control does not rescue
expression of either QE reporter gene, endogenous vg
expression or wing pouch development (G,H; red stain in G is non-specific
tracheal staining). Here, and in the remaining figures, all discs are from
mature third instar larvae, anterior is left, dorsal is up, protein or
reporter gene stains are indicated by color and relevant genotypes are
indicated above each image.
|
