spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Tub{alpha}1>vg clones act at short range to induce vg and QE reporter expression in neighboring UAS-Nrt-wg clones. (A-D) ap0 discs containing Tub{alpha}1>vg clones (black by absence of DsRed, left column), C765-Gal4/UAS-Nrt-wg clones (yellow by coincident expression of Flu-tagged Nrt-Wg, green, and DsRed), and `double' Tub{alpha}1>vg C765-Gal4/UAS-Nrt-wg clones (green by expression of Nrt-Wg in the absence of DsRed) are shown stained for Vg (A,C) or 1XQE-lacZ (B,D) expression (blue). (A,B) Double clones (green) within the prospective Drosophila wing pouch (A,B, 2) show peak expression of Vg (bright blue) and 1XQE-lacZ and induce peak expression in adjacent cells outside of the clone (note that the green territories are nested inside the larger bright blue territories, right column). Tub{alpha}1>vg clones (A, black, 1) show only moderate Vg expression (derived only from Tub{alpha}1>vg, dull blue). (C,D) Tub{alpha}1>vg clones (1, black) that abut C765-Gal4/UAS-Nrt-wg clones (3, yellow) induce cells in the latter to express peak levels of Vg and 1XQE-lacZ. The induction is not limited to those C765-Gal4/UAS-Nrt-wg cells that abut the Tub{alpha}1>vg clone, but propagates over many cell diameters into the C765-Gal4/UAS-Nrt-wg clone. Vg and 1XQE-lacZ expression are also upregulated in the Tub{alpha}1>vg cells that abut the C765-Gal4/UAS-Nrt-wg clone.





Right arrow Return to article