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Fig. 4. 5XQE transgene expression requires the vg-dependent
feed-forward signal. (A) vg0 disc carrying two
types of clones: Tub
1>vg clones, black by the
absence of GFP (red) and 5XQE>vg clones, black within the
prospective Drosophila wing pouch (outlined by dotted line) by the
absence of CD2 expression (green). Here (and in B,C), expression of the
5XQE>vg transgene is monitored indirectly in
Tub1>GFP>vg tissue by robust expression of
1XQE-lacZ (appears lavender). As illustrated at the bottom, the
Tub1>vg clone (green) has induced adjacent cells
in the abutting 5XQE>vg clone (lavender) to express the
5XQE>vg transgene, and induction of the 5XQE>vg
transgene has propagated through the clone and induced
5XQE>CD2>vg expression (yellow) in neighboring cells on the
other side. Expression of both the 1XQE-lacZ and
5XQE>CD2>vg transgenes are rescued within the
Tub1>vg clone (as in
Fig. 1F,G). (B,C)
vg0 discs that carry abutting
Tub1>vg and 5XQE>vg clones, as in A,
except that the 5XQE>vg and Tub1>vg
clones are black by, respectively, absence of GFP (green, owing to excision of
a >Tub1-GFP> Flp-out cassette) and absence of
DsRed (red). As in A, the Tub1>vg clones in both
discs (green in the diagram) have induced 5XQE>vg expression
(lavender) that propagates into the abutting 5XQE>vg clones,
rescuing wing development.
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