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Fig. 5. Control of Drosophila wing growth mediated by the 5XQE element. (A-D) vg⁰ BE-vg^GFP 1XQE-lacZ discs, either lacking (A) or carrying the 5XQE>CD2>vg (B,C) or 5XQE>Tub1-GFP>vg (D) transgene, and either lacking (A,B) or bearing (C,D) early-induced 5XQE>vg clones. 5XQE>vg clones derived from the 5XQE>CD2>vg transgene (C) are black within the prospective wing pouch by absence of CD2 (red) coupled with 1XQE-lacZ expression (blue), which is strongly upregulated in cells in which the 5XQE>vg transgene is active. 5XQE>vg clones derived from the 5XQE>Tub1-GFP>vg transgene (D) are black by the absence of GFP (green); they express 1XQE-lacZ, as in C, when located within the rescued wing pouch. BE-vg^GFP expression (green) is only barely and sporadically detectable along the D-V boundary of vg⁰ BE-vg^GFP 1XQE-lacZ discs (A), typically yielding small, anterior and posterior patches of wing tissue, encircled by rings of Wg expression (purple) in the hinge primordium. Addition of the intact 5XQE>CD2>vg transgene (B) yields detectably stronger BE-vg^GFP expression and is associated with weak, local expression of 1XQE-lacZ, possibly owing to the contribution of cryptic, low-level Vg derived from the added transgene. 5XQE>vg clones generated in this background (C,D) show significant rescue of wing growth, as visualized by the expanded domains of 1XQE-lacZ-expressing cells. They also induce neighboring cells outside the clone to activate the 5XQE>CD2>vg transgene (red). (E-H) Same as in A-D, except for the added presence of one copy of the rp49-vg transgene, which largely rescues BE-vg^GFP expression along the D-V boundary (E) and augments the weak, local activity of both the 1XQE-lacZ and 5XQE>CD2>vg transgenes in the absence of 5XQE>vg clones (E,F, compare with A,B). 5XQE>vg clones in this background cause dramatic expansions in wing growth (G,H), approximating to or exceeding that normally observed in wild-type discs, and are associated with local induction of the intact 5XQE>CD2>vg transgene in neighboring cells outside the clone (arrows). Note that 1XQE-lacZ expression is confined to the prospective wing pouch, demarcated by the inner ring of Wg (purple, arrow in H), even though the clone extends into the proximal hinge territory, indicating an independent limit to the propagation of 5XQE>vg expression.

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