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Fig. 6. Enhanced potency of the QE response increases wing growth.
(A-D) 5XQE>vg clones in wild-type Drosophila
discs, black by the absence of CD2 (green, A,D), and by robust expression of
1XQE-lacZ (red, A,B) or Vg (C, blue). The clones are associated with
expansion of the 1XQE-lacZ-expressing (wing) tissue, apparently at
the expense of the rn-only domain, normally circumscribed by an inner
and outer fold (dotted white and yellow lines, B-D, as indicated by the
arrows). The disc in B is counterstained with Wg (blue) and uniform expression
of Hsp70-GFP (green) to visualize the folds; the outer fold
correlates with the inner ring of Wg expression. (D) Dll expression
(summed staining for Dll and a Dll-lacZ transgene, blue) is not
expanded within such 5XQE>vg clones. (E) Same as in D,
except for the added presence of ubiquitous, ectopic Wg, driven under
C765-Gal4/UAS control. Dll expression now extends into the
expanded domain of wing tissue (marked by 5XQE-DsRed expression,
red).
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