DEV02874 Supplementary Material

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• Supplemental Figure S1 -

  Fig. S1. Expression of β-actin in the embryonic organ of Corti and development of the organ of Corti after transient exposure to Fgf17. (A-F) Expression of β-actin in the embryonic organ of Corti. (A-C) β-actin (red) expression is first observed at E17 and initially colocalizes with p75^ntr (green) in the pillar cells. (D-F) At P0, expression of β-actin is still present in the pillar cells but has also expanded to include Deiters’ cells. Expression of p75^ntr (green) is still largely restricted to pillar cells. Merge (F) illustrates strong overlap (yellow) in pillar cells. Scale bar: 20 μm. (G-J) Development of the organ of Corti after transient exposure to Fgf17. Lumenal surfaces of E14 cochlear explant cultures maintained in either control media or in media containing Fgf17 for the times indicated. All explants were fixed after 6 DIV. Hair cell stereocilia and cell boundaries are illustrated in green and p75^ntr is shown in red. (G) Explant treated with Fgf17 continuously. The number of OHCs is markedly reduced and there are high levels of p75^ntr expression in the OHC region. (H) Explant treated for 4 days with Fgf17, followed by a 48-hour recovery period. First and second row OHCs have begun to develop. (I) Explant treated with Fgf17 for 3 days followed by a 3-day recovery period. Complete first and second rows of OHCs are present, and p75^ntr expression is being downregulated throughout the outer hair cell region. (J) A control explant maintained 6 DIV with no exposure to Fgf17. The normal pattern of IHCs, PCs and OHCs is present. p75^ntr expression is restricted to PCs with low levels of expression in the Hensen’s cells.