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Fig. 5. Overexpression of Fgf8 induces an increase in p75^ntr-positive cells. (A) Low-magnification image of a mouse cochlear explant transfected with a control vector expressing GFP (green). The sensory epithelium (SE) is indicated by expression of p75^ntr (red) in the PCs. Kolliker's organ (KO) is located medially to the SE. (B) Similar view as in A, from an explant transfected with an Fgf8 and GFP-expressing vector (green). p75^ntr-positive PCs appear normal except in regions located near a cluster of Fgf8-transfected cells (arrows). (C) High-magnification view of a control-transfected cochlea with a large number of transfected cells. (D) High-magnification view of an Fgf8-transfected cochlea. Note the increased number of p75^ntr-positive cells. (E) Quantification of effects of expression of GFP or Fgf8 (green pixels) on p75^ntr expression (red pixels); see text for details. Control data represent regions with no electroporation in comparison to regions with high GFP or Fgf8 and GFP electroporation. Error bars indicate s.e.m. ^*, P<0.01. (F) A scatter plot of the relationship between the overall level of transfection (% green pixels) and the overall increase in p75^ntr expression (% red pixels). Best-fit linear regression line and correlation coefficient (R²) value are indicated. Scale bars: 100 µm in A,B; 20 µm in C,D.

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