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Fig. 6. Flow cytometric analysis of bone marrow, spleen, thymus and blood from
mice reconstituted with Mpl-/- E11.5 AGM. Twenty weeks
after injection, primary reconstituted mice were sacrificed and cell
suspensions prepared from femoral marrow, spleen and thymus. A peripheral
blood sample was also taken and treated as described in Materials and methods.
Bone marrow and spleen cells were co-incubated with Ly5.2 antibody coupled to
phycoerythrin (PE) and CD34, Sca1, Thy1, Ter119, Gr1 or B220 antibodies
directly or indirectly coupled to FITC. Thymus cells were co-incubated with
Ly5.2-PE and CD4-FITC or CD8-FITC. Blood cells were co-incubated with Ly5.2
and B220. Percentages of double-positive cells are indicated in the upper
right quadrant.
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