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Figure 5


Fig. 5. The effect of endoglin deletion on in vitro vascular sprout formation in differentiating EBs. (A) FACS analyses of day 6 Eng+/+ and Eng-/- EBs for VE-cadherin/FLK-1 (top) and VE-cadherin/TIE-2 (bottom). Fluorescence intensity for VE-cadherin is indicated on the y axis and for FLK-1 or TIE-2 on the x axis. (B) Percentage of EBs in each of the four classes of vascular sprout development in Eng+/+, Eng+/- and Eng-/- ES cells. Categories: I, no sprout formation; II, few sprouts; III, many sprouts but no network; and IV, many sprouts with network. Error bars indicate standard deviations from two independent experiments performed in duplicate. *, P<0.0001 versus controls (+/+ and +/-) on category IV. (C) Morphologic aspect of sprouting in Eng+/- (upper panels) and Eng-/- (lower panels) EBs.





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