|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. LvAlx1 is required in mesomere descendants for transfating.
(A) Experimental protocol. (B-G) Embryos were immunostained with
mAb 6a9 and the same embryos were photographed using brightfield (B-D) and
epifluorescence (E-G) optics. (B,E) Animal cap isolated at the 16-cell stage
and cultured for 24 hours in the absence of LiCl. The embryo lacks mesoderm
and endoderm and no 6a9-positive cells are present. (C,F) Animal cap treated
with 50 mM LiCl for 3 hours after the operation and cultured for 24 hours.
Endoderm and mesoderm have formed, including many 6a9-positive cells. (D,G)
Animal cap isolated from a LvAlx1 MO-injected embryo, treated with 50 mM LiCl,
and cultured for 48 hours. Although a gut (arrow) has formed, no 6a9-positive
cells are present. (H) RT-PCR experiments showing that animal caps
treated with 50 mM LiCl express Lvalx1 and one of its downstream
targets (Lvp16). Each experiment was repeated three times
(R1-R3).
|
