|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Overexpression of LvAlx1 is sufficient to increase numbers of
skeletogenic cells. (A) Projection of a z-stack of confocal images
of a 6 hour (early blastula) embryo expressing LvAlx1-GFP. LvAlx1 protein
accumulates in the nuclei of all blastomeres. (B) Projection of a
z-stack of the vegetal plate of a mesenchyme blastula-stage embryo expressing
LvAlx1-GFP. Cells throughout the vegetal plate have tagged protein in their
nuclei. (C-E) Embryos expressing wild-type or mutant forms of LvAlx1,
fixed at the late gastrula stage and immunostained with mAb 6a9. (C) Embryo
injected with Lvalx1.STOP mRNA (0.75 mg/ml). Numbers of 6a9-positive
cells are similar to those observed in uninjected sibling embryos
(Fig. 6A and
Fig. 8). (D,E) Lateral and
vegetal views of two different embryos injected with Lvalx1.WT mRNA
(0.38 mg/ml). These embryos have large numbers of 6a9-positive cells (100-120
cells) arranged in a radially symmetrical band. (F) Lvp16
expression (arrow) in PMCs of a control embryo. (G) A sibling embryo
injected with Lvalx1.WT mRNA (0.38 mg/ml). Overexpression of LvAlx1
leads to increased numbers of Lvp16-expressing cells, which are
arranged in a circumferential belt (arrow).
|
