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Figure 10


Fig. 10. Rescue of Drosophila Wnt5 LOF MB defects using different MB GAL4 lines. All the lines analyzed are in the Wnt5400/Y genetic background. In the x-axis label, both the GAL4 line used and the absence (-) or presence (+) of the UAS-GFP and UAS-Wnt5+ constructs are specified. n, the number of brains analyzed. The y-axis shows the percentage of wild-type brains. MB morphology was assessed using the anti-FASII antibody, or directly with GFP fluorescence when possible. P-values were obtained after {chi}2 test comparisons. The three MB GAL4 lines (OK107, 247, c739) were able to rescue the Wnt5 MB phenotype. These results indicate that the Wnt5 MB defects are linked to the lack of expression of the WNT5 protein in intrinsic MB neurons.





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