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Fig. 3. Specificity of the anti-LIO antibody. Wild-type (A) and
lio2/drlR343 (protein null) mutant (B)
Drosophila third instar larval brains were double stained with
anti-LIO (green) and anti-FASII (red). Panels A and B are reconstructions of
three confocal sections of 1 µm. No crossreaction with another protein is
detected in null individuals. Some background staining may occur in the null
individuals, but its localization and aspect are clearly unrelated to the true
labeling seen in the wild type. The wild-type left hemisphere is somehow
twisted, and its MB is not in the same focal plan, but it appears normally in
subsequent confocal sections. Scale bars: 100 µm.
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