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Figure 6


Fig. 6. DRL expression pattern in the Drosophila third instar larval brain. (A-E) Reconstructions of ten confocal sections spaced by 1 µm, with A being the most frontal section and E the most caudal. (F) A single confocal section at the level of the interhemispheric glial cells. The DRL protein (green) is expressed in the third instar larval brain in a complex pattern characterized by a lack of colocalization with MB axons well labeled by FASII (red in B). Different populations of cells, presumably neurons, send fibers crossing the brain commissure (arrow in B). About four cells (two are marked with arrowheads in F), which are glial cells (compare to A-C in Fig. 8) located in the posterior part of the brain, display cytoplasmic processes that wrap around the MB lobes (see arrow in C). DRL expression is also observed in the optic lobes (arrowhead in A-E) and in the incoming photoreceptor axons (asterisk in A-C). In the thoracico-abdominal part of the CNS, DRL protein is detected in commissural neurons (small arrows in C-E). Scale bar: 100 µm.





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