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Fig. 2. Phenotypic effects of loss of er-family function on ovule development. (A-D) Scanning electron micrographs (SEMs) of Arabidopsis ovules at anthesis. (A) Wild type; (B) er-105 erl1-2/+ erl2-1; (C) er-105 erl1-1 erl2-1/+; (D) er-105 erl1-2 erl2-1/+. In er-105 erl1-2 erl2-1/+, both inner and outer integuments are shorter leaving the nucellus exposed (arrows). (E,F) Sagittal sections through the gametophyte of a er-105 erl1-2/+ erl2-1 (E) and er-105 erl1-2 erl2-1/+ (F) ovule at anthesis. In the er-105 erl1-2 erl2-1/+ mutant, the gametophyte has degenerated into a mass of small cells. (G) SEM of a developing er-105 erl1-2 erl2-1/+ stage 2-I ovule. Both the inner and outer integument initiate as smooth rings at the base of the nucellus, with the outer integument initiating asymmetrically as in wild type. (H-K) In situ hybridization for (H,I) PHB and (J,K) WUS expression. No difference in PHB or WUS expression is detected in mutant ovules, indicating normal regional specification. No signal was detected with PHB or WUS sense probe. f, funiculus; es, embryo sac; ii, inner integument; oi, outer integument; n, nucellus. Scale bars: 20 µm.

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