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Fig. 4. Molecular cloning and gene structure of Ppa-pax-3, and the
alignment of PAX-3-type proteins. (A) Map position of
Ppa-gev-2 (Ppa-pax-3) on chromosome II close to the
molecular marker S98. The three BAC clones 1-E10, 17-D02 and 17-O15 define a
physical interval for the gene. (B) Protein domain structure of
Ppa-pax-3. Ppa-pax-3 contains a bona-fide paired domain (PD) with the
PAI and RED subdomains, as well as an octapeptide and a homeodomain (HD).
(C) Ppa-pax-3 cDNA sequence as obtained from 5' and
3' RACE experiments. Conceptual translation starts with the first
in-frame ATG codon after the SL1 splice acceptor site. Introns are indicated
by black triangles. The breakpoints of the C-terminal deletion of
tu214 are indicated by arrowheads. The mutation of tu358
results in an amino acid replacement from His to Arg and is indicated by an
arrow. The PD domain is boxed, the octapeptide is underlined and the HD is
highlighted. (D) Amino acid comparison between P. pacificus, C.
elegans, mouse and human PAX-3-type proteins, indicating a strong amino
acid sequence conservation of all protein domains.
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