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Figure 5


Fig. 5. smedinx-11(RNAi) gradually depletes the neoblast compartment. FACS profiles using Hoechst blue versus calcein, highlighting X1 (blue rectangle), X2 (red rectangle) and Xins (yellow rectangle) subpopulations from dissociated control planarians (left-most), planarians at 7 days post-irradiation (6000 rad; irradiated), and at 10 and 14 days after smedinx-11(RNAi). Notice that the numbers of dividing X1 neoblasts were sharply reduced after irradiation and at 10 days after smedinx-11(RNAi), but this did not occur for X2 cells (see Fig. 4E for quantification). However, as the phenotype progressed, the X2 cells were reduced to levels comparable to irradiated worms >14 days after dsRNA smedinx-11 exposure (data not shown).





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