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Fig. 3. Fluorescent double in situ hybridizations showing expression of Coll
IX, Coll II and Opn relative to each other and to the
domains of Runx2, PTHrP-R, Bmp4 and Bmpr1b
expression. (A-C) Direct comparison of the Opn and
Coll IX expression domains. (C) There are cells that express Coll
IX only (red; broad arrows), Opn only (green; arrowheads) or
that co-express Opn and Coll IX (yellow; narrow arrows).
(D-F,J-O) Considerable overlap is detected between Opn
and Bmp4 (D-F), between Runx2 and Coll IX (J-L),
and between Coll IX and PTHrP-R (M-O). (G-I)
Expression domains of Opn (green) and Runx2 (Cbfa1;
red) have very little or no overlap. The same is true for the expression
domains of Coll II versus BspII (S-U) and
Ihh versus Opn (V-X). Some overlap is detected for
the expression domains of Bmpr1b versus Ptc1 (P-R).
Scale bar: 200 µm.