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Figure 6


Fig. 6. Molecular and histological analysis of RCAS::Ihh and RCAS::PTHrP-infected frontal bones. (A-P) Analysis of the RCAS::Ihh-infected frontal bones with molecular markers and H&E staining shows that infected bone failed to produce differentiated osteoblasts (I,J) and to mineralize (M-P). Framed areas in M and N are shown amplified in O and P, respectively. (Q-F1) Misexpression of RCAS::PTHrP resulted in a very similar phenotype, with a loss of Opn expression in the infected skeletal element (Y,Z). Framed areas in C1 and D1 are shown amplified in E1 and F1, respectively. Scale bars: 1 mm.





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