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Fig. 7. Analysis of 18.5 dpc Ihh-/- mutant mouse
embryos. The skull of wild-type (WT) embryos (A-C) is larger than
that of mutant siblings (D-F). Notice the presence of the ossified
basal cranial bones in the mutant. Arrows indicate the leading edge of the
mineralized dermal calvarial bone. Expression of the early osteoblastic
markers, such as Runx2 (G) and Osx (H), is
severely reduced in the Ihh-/- condition (K and
L, respectively). Red brackets indicate area occupied by
undifferentiated proliferating osteoblasts. The expression of Opn
(I,M), a marker for more mature osteoblasts, is unaltered or
slightly expanded in the mutant condition. Green brackets indicate area
occupied by differentiated mature osteoblasts. (J,N) H&E
stainings of wild-type and mutant dentary bones, respectively. bas,
basisphenoid; boc, basioccipital; dnt, dentary; exo, exoccipital; fnt, frontal
bone; int, interparietal; max, maxillary; MC, Meckel's cartilage; nas, nasal;
pal, palatal; par, parietal; pte, pterygoid; o.t., os tympanicum. Scale bars:
1 mm in A; 200 µm in G.
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