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Fig. 3. Quantitative representation of the capacity of all PSM explant types
treated with pancreatin or collagenase to form somites. The average number
of somites formed for each explant type and enzyme treatment is depicted, and
the proportion of explants forming 0, 1, 2, 3, 4 and 5 somites during the
6-hour culture period is indicated with a colour code. Note that the somites
formed in PSM and PSM+endoderm explants isolated with pancreatin (marked with
) were frequently less compacted and had incomplete clefts
as compared with all other experimental situations. A factorial ANOVA (results
depicted at the bottom of the figure) followed by a contrast analysis of
predicted differences (results shown in graph) were performed to test for
differences among treatments. ns, not significant. Error bars represent 95%
confidence limits.