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Figure 8


Fig. 8. A 70 kDa peptide containing the fibronectin assembly domain inhibits somitogenesis in embryo explants cultured for 12 hours. (A-D) Ventral view of chick embryo explants cultured with BSA (control) or the 70 kDa fibronectin fragment. Endoderm was removed on the left while the other side was kept intact. Embryos incubated with BSA form the expected number of somites in 12 hours (A,C), whereas embryos cultured with the 70 kDa fibronectin fragment form fewer somites (B,D). When embryos are cultured with 100 µg/ml of the peptide, somitogenesis is practically abolished (D). (E) Bar chart of the results obtained in A-D. Error bars represent 95% confidence limits. *, Somites formed in culture; BSA, bovine serum albumin; 70 kDa, 70 kDa fibronectin fragment; ns, not significant; **, P≤0.005. Scale bars: 100 µm.





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