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Fig. 2. Microarray analysis of gene expression during epithelial differentiation
in rat metanephric mesenchyme. (A) Heatmap representation of all
genes upregulated independently by Lif, NGAL and NHBF in a temporal sequence
from day 0 (freshly isolated mesenchyme) to day 7 (differentiated renal
epithelia). Average profiles (right) were deduced arithmetically and grouped
into three classes based on the time point of peak expression (class A, day
1-2; class B, day 3-5; class C, day 7). Note that only genes upregulated by
all three inducers were included in this analysis. (B) Correlation
matrices comparing global gene expression at individual time points (day 0 to
day 7) were calculated and hierarchical clustering identified time points of
major shifts in gene expression (tree diagram). Pearson correlation
coefficients R of neighboring time points (blue) illustrate the
degree of change in global gene expression. Epithelial differentiation is
depicted at the times of peak expression of each class of genes.
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