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Fig. 5. Pds5B-deficient mice manifest germ cell defects.
GCNA1 staining of germ cells of WT (A) and Pds5B-/-
(B) neonatal ovaries, and WT (C) and
Pds5B-/- (D) neonatal testes show reduced germ
cells in Pds5B-/- mice. Arrows point to empty testicular
cords in D. (E,F) GCNA1 immunohistochemical analysis of E16.5 germ
cells in WT (E) and Pds5B-deficient (F) testes shows a reduction in
germ cells during embryogenesis. (G,H) GCNA1 staining of
two-week testes transplants of E16.5 wild-type (G) and
Pds5B-/- mice (H). In contrast to the presence of germ
cells in the periphery of WT seminiferous tubules (G), note complete loss of
germ cells in Pds5B-/- testis explant (H).
*Non-specific auto-fluorescence signal; red, GCNA1; blue,
bisbenzimide. (I) Quantitative analysis of germ cells in E16.5 testes
of WT and Pds5B-deficient mice (n=4 for each genotype).
Error bars represent SEM. *P<0.001, Student's unpaired
t-test. (J) Proliferation index measured by BrdU incorporation
in GCNA1-positive germ cells at E12.5 (n=3 for each genotype and 100
GCNA1-positive cells were counted for each animal). Error bars represent
s.e.m. *P<0.05, Student's paired t-test. Scale
bars: 50 µm.
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