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Fig. 6. Pds5B-/- MEFs lack sister chromatid cohesion
defects. (A,B) Metaphases from mouse embryonic fibroblasts
(MEFs) derived from WT and Pds5B-/- mice show no
differences in sister chromatid cohesion. (C) Multi-species alignment
of AT hook domains of PDS5B. Hs, Homo sapiens; Pa, Pan troglodytes; Ca,
Canis familiaris; Mm, Mus musculus; Rn, Rattus norvegicus; Ga, Gallus gallus;
Xe, Xenopus tropicalis; Da, Danio rerio; Te, Tetraodon nigroviridis.
(D) Subcellular localization of human homologs of PDS5 using
EGFP-tagged PDS5A and PDS5B fusion proteins. HeLa cells were transfected with
EGFP-PDS5A and EGFP-PDS5B using Lipofectamine. At 24 hours later, the EGFP
signals were examined. Left panels are phase-contrast images of HeLa cells;
right panels are images of GFP signals. Note that EGFP-PDS5B is present in the
nucleus and highly enriched in the nucleolus (arrows), whereas EGFP-PDS5A is
localized to the nucleus but excluded from the nucleolus (arrows). Scale bar:
5 µm.
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