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Fig. 2. Lmx1a regulates mesDA neurogenesis by inducing proneural factor
expression and cell growth in their progenitors. (A) The mesDA
number (indicated by Th and Lmx1b expressions) is reduced in dreher mutant
embryos compared with wild-type embryos at E13.5, but the fate of
Lmx1a+ neurons is not affected. The intensities of anti-Th and
anti-Lmx1b staining were lower in dreher mutants. However, almost all
Lmx1a+ neurons and neurons generated from ventral midline regions
of the mesencephalon, in mutants and wild-type controls, expressed Lmx1b.
(B) Neurogenesis in Lmx1a+ progenitors is affected by the
dreher mutation. The Th+ neuron number is severely reduced in
dreher mutants at E11.5. Ngn2 and Mash1 expression levels in the
Lmx1a+ VZ region are reduced and, consistently, the generation of
Lmx1a+ TuJ1+ neurons is less efficient in mutants. Note
that reductions in the expression levels of proneural factors are apparent in
the medial part of the Lmx1a+ domain. (C) Quantification of
Lmx1b+ neurons in the ventral mesencephalon of wild-type,
heterozygous and homozygous dreher mutant embryos at E13.5. (D)
Quantification of BrdU-incorporating, Ngn2+ and Mash1+
cells in Lmx1a+ VZ region.
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