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Figure 5


Fig. 5. FACS isolation of mesencephalic ventral midline cells with FP-like characteristics. (A) Corin is selectively expressed in ventral midline cells from the mesencephalon to the spinal cord of E12.5 mouse embryos. (B) Co-expression of Corin and FP4 in E13.5 rat FP cells. Note that Corin is expressed only in medial FP cells in the spinal cord. (C) FACS analysis of E13.5 rat ventral mesencephalon and metencephalon cells. Corin+ cells can be sorted with more than 95% purity. (D) Rat mesencephalic Corin+ cells express Lmx1a, FP4, nestin but not TuJ1. Virtually all the Corin+ cells express Lmx1a and nestin. FP4 expression is detected in more than 95% of the Corin+ cells. (E) Mesencephalic Corin+ cells express Shh, Hnf3ß and Ntn1 transcripts. G3PDH, glyceraldehyde-3-phosphate dehydrogenase.





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