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Fig. 2. No interaction is required between the left and right Vp motor axons for
projection to the bilateral interhyal muscles in BA2. (A)
Time-lapse images from 48-72 hpf of the Vp motoneurons after laser irradiation
to the lateral part of the Vp motoneurons on the right side (a-e,
arrowheads). Dorsal (a-e) and ventral (f-j) views of the same operated
zebrafish embryo. Anterior, top. Brackets indicate remaining cells on the
right side (a-e). Asterisks indicate the nerve end of the operated motoneurons
(f-j). (B) Anterograde (a) and retrograde (b) labelling
of the Vp motor axons of 72-hpf wild-type embryos with DiI. Ventral view;
anterior, top. Asterisks indicate the points of DiI application. Arrowheads
indicate the ends of the labelled axons. (C) Stochastic expression of
GFP using the Isl1-GFP construct. Ventral (a) and dorsal (b)
views; anterior, top. Arrowheads indicate a single Vp motoneuron labelled with
the Isl1-GFP construct (a) and the ends of the labelled axons (b).
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