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Fig. 1. Comparisons between X-Gal staining patterns in Wnt reporter mice.
(A-D) X-Gal staining for Wnt reporter activity at E9.5. (A) Staining is
evident in the midbrain (mb) and anterior neural folds (nf) of BATgal embryos.
(B) A lateral view shows reporter activity in the first pharyngeal arch (p1).
(C) In TOPgal embryos, Wnt reporter activity is evident in the first
pharyngeal arch (p1) as well as the midbrain (mb) and hindbrain (hb). No Wnt
reporter activity is detectable in the anterior region of the face (f). (D)
Wnt reporter activity in the midbrain (mb) and anterior neural folds (nf) of
TOPgal embryos generally mirrors that seen in BATgal embryos. Note that the
BATgal and TOPgal embryos are at slightly different stages of development, as
gauged by the extent of neural tube fusion (dotted white lines, A and D).
(E-H) X-Gal staining at E10.5. (E) Wnt reporter activity is evident in
the midbrain (mb) and forebrain (fb) of BATgal embryos. (F) Reporter activity
is also evident in the distal regions of the maxillary (mx) and mandibular
(mn) prominences, the aboral surface of the mandible (red arrow), the proximal
first arch (red arrowhead), the lateral nasal (l) prominences, and around the
developing eye (e). (G) A nearly identical pattern of reporter activity is
seen in the TOPgal face. (H) One subtle difference in reporter activity at
this stage is in the forebrain region, where X-Gal staining appears more
restricted than that observed in BATgal embryos (compare E with H).
(I-L) X-Gal staining at E14.5. (I) Wnt reporter activity is evident in
surface ectoderm overlying the dermal papilla, signaling the site of whisker
development. (J) At the same stage, Wnt reporter activity is abundant in the
maxillary (mx) prominences but absent in the frontonasal (f) prominence. (K,L)
The pattern of Wnt reporter activity in the TOPgal face and whisker primordia
mirrors that seen in BATgal embryos at the same stage.
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