DEV010140 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. GATA-a and NK-4 expression in the TVCs requires Ets1/2-mediated FGF signalling. (A-E) Embryos electroporated with Mesp>lacZ and indicated constructs were stained for GATA-a (A,C,E) or NK-4 (B,D) mRNAs (green) and beta-galactosidase (red). (E) GATA-a expression persists in non-migrating TVCs after Mesp:VP16 overexpression (white arrowhead). Numbers refer to embryos showing the depicted phenotype. (F) Proportion of embryos showing GATA-a or NK-4 expression in the B7.5 lineage after Mesp:VP16 or FoxF:WRPW expression. Note that GATA-a and NK-4 expression persist in 50% and 36% of embryos expressing FoxF:WRPW, respectively. (n=total number of embryos analyzed by confocal microscopy.)

• Movie 1 -

  Movie 1. Wild-type juvenile heart.

• Movie 2 -

  Movie 2. Ets:VP16/FoxF:WRPW juvenile heart.