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Fig. 2. SULF1 and SULF2 are differentially expressed in the embryonic
esophagus. (A-G) SULF1 esophageal expression at E14.5.
Sulf1 mRNA (B) and protein (C) were detected at the outer layer of
the esophagus and the protein was mostly on the cell surface (insert in C).
The outer layer of the esophagus expresses SM22, a smooth muscle marker (D).
Immunostaining with the rabbit anti-MS1HD antibody and the rabbit anti-SM22
antibody together identified SULF1 on the membrane of SM22-expressing cells
(E); the insert shows a magnified image of the outlined staining. SULF1 did
not colocalize with the TuJ1 staining (F). GDNF was detected diffusively
across the esophageal muscle layers and partially overlapped with SULF1 in the
outer layer (G). (H-J) SULF1 expression and colocalization with GDNF in
the esophageal muscle layers at E16.5 by double staining. (K-N) SULF2
esophageal expression at E14.5. Cross-sections were double stained with SULF2
and TuJ1 antibodies. SULF2 tightly associated with TuJ1 staining. Scale bars:
100 µm.
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