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Fig. 6. Porcupine/palmitoylation regulates Wnt-induced proliferation in the
chick neural tube. Embryos were electroporated in the neural tube as
described in Materials and methods. GFP-positive embryos were harvested for
analysis 24 hours post-electroporation. Embryos were fixed, cryosectioned and
immunostained with anti-phosphohistone H3 (red), which marks cells in late
G2/M phase. (A) A section from a representative embryo electroporated
with mPorcD. The right side of the neural tube has been electroporated
(experimental), whereas the contralateral side serves as a control. (B)
The ratio of experimental/control proliferation indices for embryos
electroporated with Wnt1, Wnt3a, mPorcD, mPorcD(H341Q), Wnt1(C93S) and
Wnt3a(C77S). Error bars show the standard error. N, number of embryos analyzed
for each data point; n, number of sections analyzed. *,
Significantly different from the control (P<0.05). nt, neural
tube; no, notochord.
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