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Fig. 8. Ectopic expression of porcupine in the chick neural tube shortens the
BAT-gal reporter gradient. The BAT-gal reporter construct was
co-electroporated into chick neural tubes along with either pCIG (control),
Wnt1, Wnt3a, mPorcD or mPorcD(H341Q) constructs. GFP-positive embryos were
harvested for analysis 24 hours post-electroporation. Embryos were fixed,
cryosectioned and immunostained with antibodies against ß-galactosidase.
Sections were imaged with confocal microscopy. Merged images of the GFP and
ß-galactosidase expression are shown above, separate channels below. The
vertical green line demarcates the distance of GFP expression from the
dorsal-most extent of the neural tube; a red line demarcates the distance of
BAT-gal expression from the dorsal neural tube. A graphic representation of
the ratio of the BAT-gal distance divided by the GFP distance is shown. N,
number of embryos analyzed; n, number of sections analyzed. Error bars
indicate standard error.
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