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Fig. 7. P4H1 overexpression reduces the O2 requirement for
culmination in Dictyostelium. (A) Western blot analysis of
P4H1-myc levels in clones of strain Ax3 transfected with the promoter
expression constructs described in Fig.
5A. (B) Effects of overexpressing P4H1 in the Ax3
background on the hypoxic blockade, as determined by counting
detergent-resistant spores. Spore numbers correlated with morphological
culmination (not shown). Results are typical of three independent trials.