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Figure 7


Fig. 7. P4H1 overexpression reduces the O2 requirement for culmination in Dictyostelium. (A) Western blot analysis of P4H1-myc levels in clones of strain Ax3 transfected with the promoter expression constructs described in Fig. 5A. (B) Effects of overexpressing P4H1 in the Ax3 background on the hypoxic blockade, as determined by counting detergent-resistant spores. Spore numbers correlated with morphological culmination (not shown). Results are typical of three independent trials.





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