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Fig. 2. Premature neural differentiation of the epiblast occurs in
Bmpr1a-/- embryos. (A,B) At 5.5 dpc,
Oct4 expression is observed throughout the epiblast of control and
Bmpr1a mutant mouse embryos (n=5). (C,D) At
6.5 dpc, Oct4 is expressed in the epiblast of controls but its
expression is downregulated in 50% of Bmpr1a-/- embryos
(n=4/8). (E,F) Nanog expression is observed in
the proximal posterior epiblast at 6.5 dpc in controls, but not in
Bmpr1a mutant embryos (n=6). (G,H) At 6.5 dpc,
Fgf5 is expressed throughout the epiblast of controls but is strongly
downregulated or absent in Bmpr1a-/- embryos
(n=5). (I-L) At 7.5 dpc, Six3 and Hesx1 are
expressed in the anterior neural ectoderm of controls, but in Bmpr1a
mutant embryos expression of both these genes is observed throughout the
epiblast (n=7/8 and n=4/6, respectively).
(M,N) Sox1 expression is not observed at 7.5 dpc in
control embryos, but in Bmpr1a-/- embryos expression is
observed in the entire epiblast (n=7/8). (O,P) No
Foxg1 expression is observed in control embryos at 7.5 dpc, but in
Bmpr1a-/- embryos Foxg1 is expressed in a
discrete patch of cells at the boundary between the embryonic and
extra-embryonic region (n=4/6). (Q,R) At 6.5 dpc,
Six3 is not expressed in control embryos but is present throughout
the epiblast of two-thirds of Bmpr1a-/- embryos
(n=3/5). (S,T) Hesx1 is expressed in the
anterior visceral endoderm at 6.5 dpc, but in a third of Bmpr1a
mutant embryos expression is observed throughout the epiblast
(n=3/10).
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