spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Nodal signalling can resue the pluripotency defect of Bmpr1a mutant mouse embryos. (A,B) At 4.5 dpc, Nodal is expressed in the epiblast and primitive endoderm in both control and Bmpr1a-/- mouse embryos (n=5/5). (C,D) At 5.5 dpc, Nodal is expressed in the epiblast and visceral endoderm of controls but is severely downregulated in a proportion of Bmpr1a mutant embryos (n=2/3). (E,F) Cripto is expressed in the epiblast at 5.5 dpc of controls, but is lost in a proportion of Bmpr1a-/- embryos (n=4/7). (G,H) At 5.5 dpc, Gdf3 expression is observed throughout the epiblast of control and Bmpr1a-/- embryos (n=5/5). (I,J) Bmpr1a-/- embryos display self-renewal defects when cultured in serum-free conditions and Bmp4. (K,L) 20 ng/ml activin can rescue the self-renewal defects of Bmpr1a-/- embryos (n=5/7).





Right arrow Return to article