DEV005181 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. PGL-1 levels are elevated in older kgb-1 worms. (A) One arm of an extruded gonad from an N2 worm grown at 20°C for 4 days beyond L4 reacted with anti-PGL-1 antibodies. (B) The gonad in A stained with DAPI. (C) An extruded gonad from an age-matched kgb-1 mutant reacted with anti-PGL-1 antibodies. (D) The gonad in C stained with DAPI.

• Supplemental Figure S2 -

  Fig. S2. KGB-1 is uniformly cytoplasmic in the distal gonad and is found in dispersed particles in the cytoplasm of the cellularized oocytes. (A) A section of a splayed gonad (wrapped back on itself similar to Fig. 2E; 1000× magnification) of a young N2 adult raised at 20°C reacted with anti-GLH-1 antibody (rabbit). Germ cell nuclei in the distal gonad are on top, with four cellularized oocytes below. (B) The same gonad section reacted with the affinity-purified anti-KGB-1 mouse antibody (green). (C) DAPI staining of the gonad in A and B. (D) A splayed gonad of another young N2 adult raised at 20°C reacted with anti-GLH-1 antibody (rabbit), also at 1000×. (E) The same gonad as in D reacted with preimmune anti-KGB-1 mouse sera as a negative control to show antibody specificity. (F) DAPI staining of the gonad in E.

• Supplemental Figure S3 -

  Fig. S3. Input proteins for the Vasa pull-down assays in Fig. 6C. Lanes are as listed in Fig. 6C. Anti-His antibodies detected protein in all lanes; the smaller band in lanes 2 and 3 is likely to be a breakdown product.