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Fig. 2. Rab11 is required to maintain E-cadherin at the cap cell-GSC junction
and to anchor the fusome to the anterior cortex of the GSC.
(A,B) Region 1 of a mosaic Drosophila germarium
immunostained 8 days after clone induction (ACI) for E-cadherin (red),
-Spectrin (blue) and nGFP (green). (A) A 2-cell rab11-null
germline cyst is outlined (dashed line). A dividing wild-type GSC (boxed) is
shown to the left and magnified in B, where the dashed line outlines the cap
cells. Note that the plane of division (evident by the stretched-out fusome)
is such that both daughter cells remain in the niche, with one filling the
vacancy created by a lost GSC. (C-E) Wild-type (C) and mosaic (D,E)
germaria immunostained for nGFP (green), E-cadherin (red) and -Spectrin
(blue) 2 days ACI. Wild-type and rab11-null GSC-cap cell junctions
are indicated with arrowheads and arrows, respectively. E-cadherin staining is
reduced at the mutant junctions at the expense of increased fusome staining,
especially in E, where several strong dots of staining are evident. (F)
Dividing rab11 (arrow) and wild-type (arrowhead) GSCs immunostained
for -Spectrin (blue), Vasa (cytoplasmic green, germline only) and nGFP
(green) and stained with DAPI (red). The DAPI-stained nuclei at the left
correspond to cap cells. The rab11 GSC fusome is splayed and
displaced from the anterior cortex. (G) Mosaic germarium immunostained
for nGFP (green), -Spectrin (red) and Bam (blue) 2.2 days ACI. The
rab11-null GSC (outlined in yellow) exhibits only background levels
of Bam expression. A wild-type GSC and a 2-cell germline cyst (outlined in
white) are shown for comparison. Scale bars: 10 µm.
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