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Fig. 3. rab11-null germline cysts arrest early and display defects in
fusome segregation and bulk membrane trafficking. (A,B)
Mosaic Drosophila germarium immunostained for (A) 
-Spectrin
(red), Vasa (cytoplasmic green) and nGFP (green) or (B) E-cadherin (red),
-Spectrin (blue) and nGFP (green). Severely affected
rab11-null germline cysts with no detectable fusomes and reduced
numbers of germline cells are outlined in yellow. A less affected
rab11-null germline cyst with a normal fusome is outlined in white in
A and indicated with an arrow in B. (C) Wild-type germarium
immunostained for E-cadherin (red) and -Spectrin (blue). The arrowheads
point to the posterior of the oocyte, where enriched accumulation of
E-cadherin is evident, especially in the region 2B oocyte (left arrowhead).
(D) Mosaic germarium immunostained for E-cadherin (red),
-Spectrin (blue) and nGFP (green). The arrow points to a region 2B
rab11-null oocyte with greatly reduced E-cadherin accumulation
(compare with left arrowhead in C). The arrowhead points to a wild-type region
3 oocyte, where enriched E-cadherin expression is still apparent. A severely
affected, rab11-null germline cyst, similar to those seen in B, is
outlined. (E,E') Mosaic germarium immunostained for Orb
(red, oocyte) and nGFP (green). The bracketed area in E is shown at a
different focal plane in E'. Note that the oocyte is positioned at the
posterior end of the wild-type germline cysts (arrowheads), but at the center
of the rab11-null germline cyst (arrow). (F,G) Mosaic
germarium immunostained for nGFP (green) and HtsRC (red) to label ring canals.
(F) Left arrow points to a rab11-null germline cyst, with clumped
ring canals. The right arrow points to a mosaic germline cyst, where the ring
canals are only clumped in the rab11-null (GFP-negative) portion. (G)
Germarium with a completely rab11-null germline. All ring canals are
clumped in the center of the cysts. Scale bars: 10 µm.
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