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Fig. 2. Grk is mislocalized to post-Golgi compartments in Drosophila
Rab6 mutants. (A,B) Low magnification electron micrographs
of control (A) and rab6D23D mutant (B) egg chamber.
(A',A'') Magnified view of boxed area from A.
(B',B'') Magnified view of boxed area from B. nc,
nurse cells; oo, oocyte; fc, follicle cells. ER morphology in control (arrows
in A') and in rab6D23D (arrows in B'). Normal
tER-Golgi unit morphology in the control (A'') and onion-like shaped
morphology in mutant (B''). Cisternae (arrowheads) were not altered in
number, although severely swollen, in the mutant. (C) Drab6 and Grk
colocalization in GFP-Drab6-rescued oocyte. (D,E) Projection of
optical sections (
20 µm) of control (D) and
rab6D23D (E) stage 9 oocytes, stained for Grk and F-actin.
Upper insets, confocal sections at the nucleus. Lower insets, control egg and
ventralized egg in the mutant. In contrast to the control, Grk formed larger
particles close to and ring-like structures remote from the nucleus
(arrowhead) in the mutant. (F,G) Grk and Lva colocalized
marginally to small particles close to the nucleus in the control (F) and in
rab6D23D (G) oocytes. Colocalization was not observed for
the large Grk-positive ring-like structures (G, arrowheads in enlarged view
shown in inset) in the mutant. (H,I) Control and Drab6 mutant
egg chamber, respectively, labeled with Grk and LysoTracker (Lyso) to reveal
endosomal compartments and lysosomes. (H',I')
Magnified view of upper boxed area from H and I, respectively.
(H'',I'') Magnified view of lower boxed area from H
and I, respectively. Grk colocalized in a ring-like manner with Lyso-positive
structures close to and distal to the nucleus only in
rab6D23D. Asterisk, oocyte nucleus. Scale bars: 20 µm;
500 nm in B''.
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