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Fig. 4. Drab6 is actively transported along microtubules. Frames taken from time-lapse recordings of GFP-Drab6-expressing Drosophila egg chambers. Untreated (A) GFP-Drab6-rescued egg chamber and (B) colchicine-treated egg chamber. (C) GFP-Drab6-expressing egg chamber overexpressing Dynamitin and (D) Khc^7.288 germ line clone expressing GFP-Drab6. Below each panel, parameters of vesicle movement derived from the time-lapse recordings are indicated (A'-D'). Particle parameters were determined using ImageJ. Particles in oocytes and nurse cells were traced in a single optical plane in three different egg chambers for each: control, Khc clones and overexpression of Dynamitin. (A,B) Colchicine treatment abolished accumulation in the center as seen in controls (arrow) and particles seemed to form bigger clusters. (C) Overexpressing Dynamitin reduced accumulation of Drab6 at the cortex (arrow). (D) Stage 8 Khc⁷⁸⁸ oocyte expressing GFP-Drab6. Drab6 did not accumulate in the center and formed clusters close to the oocyte nucleus (asterisks). Scale bars: 20 µm.

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