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Fig. 1. Foxn4 is sufficient to induce V2b and suppress V2a
interneurons. In this and subsequent figure legends, consecutive sections
are labelled A,A',A'' etc, and different fluorescence channels of
the same micrograph are labelled A,A1,A2 etc. (A-J') Chick
embryos were electroporated at st12-14 with
ß-actin-Foxn4-IRES-GFP and harvested after 24 or 48 hours.
Expression of the vector was confirmed by in situ hybridisation (ISH) for
Foxn4 or immunolabelling for GFP (panels marked Foxn4-GFP). Foxn4
induces robust ectopic expression of Gata2 at either 24 or 48 hours
post-electroporation (A',E',F). Foxn4 induced Gata3
(D',I') and Scl (J', note ventral induction,
arrowhead) only after 48 hours. Foxn4 does not induce ectopic expression of
Chx10 (B',G,H) or Lhx3 (C',E''). On the contrary,
Foxn4 represses endogenous Chx10 in the p2 domain (G,H).
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