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Figure 6


Fig. 6. Foxn4 controls Mash1 expression. (A) Double ISH for Foxn4 (red) and Mash1 (green) in E10.5 mouse cord. Note the extensive overlap in the p2 domain. (B,B') Foxn4 induces ectopic expression of Cash1 in chick electroporation experiments. (C,D) Foxn4 expression does not depend on Mash1; there is no noticeable change in the Foxn4 ISH signal in Mash1-null mice compared with wild type. (E,E') Electroporation of ß-actin-Mash1-IRES-GFP in the st13-14 chick neural tube induces Dll4 after 24 hours. Mash1 expression was confirmed by GFP immunolabelling (E) and Dll4 by ISH (E'). (F-G) Mash1 did not induce ectopic Chx10, but repressed endogenous Chx10 V2a INs in the p2 domain. G is a magnified view of the ventral part of panel F'. (H-H'') Also, Mash1 did not induce ectopic V2b markers Gata2 or Scl. (I,J) Despite the fact that Mash1 is sufficient to induce Dll4 in chick (see E,E'), Mash1 is not required for Dll4 expression in mice; Dll4 is expressed as normal in the ventral spinal cord of Mash1-null mice.





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