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Fig. 7. Notch1 is required for specification of V2b interneurons. Mice
carrying a floxed allele of Notch1 and a Nestin-Cre
transgene (Notch1 cKO mice) were analysed at E10.5 and E11.5 by ISH
and double immunolabelling for V2a and V2b IN markers. (A-D) There is a
two-fold increase in the number of Chx10 immunopositive V2a INs in the
Notch1 cKO compared with wild type, whereas Gata3 immunopositive V2b
INs are abolished. In addition, the Chx10-positive V2a INs accumulate near the
midline of the spinal cord instead of migrating into the parenchyma.
(E,F) Double immunolabelling for Olig2 (magenta) and Hb9
(green). In the Notch1 cKO, Olig2-positive cells are missing and the
Hb9 population is similar to that in the control. Therefore, Notch1 activity
is needed for V2b IN production; in the absence of Notch1, V2b INs are
respecified as V2a INs with little or no influence on MN fate. (G-J) In
the Notch1 cKO, expression of Foxn4 is increased at E10.5
relative to wild type (compare G with H), but is almost extinguished by E11.5
(I,J). (K-N) Scl (V2b INs) is reduced at E10.5 (K,L) and
absent at E11.5 (M,N) in the Notch1 cKO. Note that the ventral half
of the central canal (and the VZ) is lost in the Notch1 cKO mouse
between E10.5 and E11.5.
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