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Fig. 5. SOX3 and SOX2 expression in the pharyngeal region in wild-type and
mutant mouse embryos. (A,E,I) Whole-mount immunofluorescence and 3D
reconstruction of embryo sagittal halves. (B-D,F-H,J-L)
Immunofluorescence on sections. (A,E) Anti-SOX3 immunofluorescence. (A)
Expression at 8 ss in the neural plate and pharyngeal region. (B-D) Transverse
section at 8-10 ss at PP1 level (section plan shown in A). (B) DAPI. (C) SOX3
is detected in the proximal pharyngeal ectoderm and underlying lateral
endoderm (note non-specific staining in foregut diverticulum); inset magnified
PP1 region boxed. (D) SOX2 is present more widely than SOX3 in the pharyngeal
endoderm. (E) At 9.5 dpc SOX3 expression is restricted to the posterior pouch
margins. (F-H) Coronal section in the proximal pharyngeal region at 9.5 dpc.
(F) DAPI. (G) SOX3 is detected in both epithelia in the posterior pouch
margin. This regionalisation is acquired as the arches develop: in the more
caudal PA3, SOX3 expression is not as restricted as in PA2. (H) SOX2 is
present in the pharyngeal endoderm and weakly in the ectoderm. (I) Anti-GFP
immunofluorescence on a Sox3 null embryo at 9.5 dpc. GFP (from the
Sox3 locus) is present across the reduced proximal region of PA2
(arrow). (J-L) Coronal section in the proximal pharyngeal region at 9.5 dpc in
a Sox3 null embryo. (J) DAPI staining (inset: magnified abnormal PA2,
boxed). (K) GFP is present all around the hypomorphic proximal region of PA2.
(L) SOX2 expression also highlights the abnormal morphology of PA2 (expression
is stronger in the ectoderm than in H as the section is more distal). Scale
bars: 50 µm in B, for B-D; in F, for F-H and J-L.
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