DEV003095 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Medial hindbrain roof plate epithelial cells do not express AP-2α, Pax3, Sox9 or Br. (A-D) Transverse sections through E11.5 hindbrain processed to detect AP-2α, Pax3, Sox9 and Br mRNA show expression of these markers is absent from the medial roof plate epithelium (hRPe, black arrows). (E-I) mRNA expression is detected in other regions of the same embryos, including neural crest cells, notochord (black brackets) and limb (red arrow). Thickness of medial hRPe varies due to sectioning angle.

• Supplemental Figure S2 -

  Fig. S2. Cre expression is limited to the dorsal hindbrain neuroepithelium of Wnt1::cre embryos. (A-C) Transverse sections through E8.5-E10.5 hindbrain processed to detect cre mRNA show expression limited to the dorsal hindbrain neuroepithelium. Wnt1::cre is not expressed in hRPe or neighboring mesenchyme.

• Supplemental Figure S3 -

  Fig. S3. Production of hindbrain choroid plexus epithelium begins at ∼E9.5. (A,B) Coronal sections processed for PLAP detection (dark precipitate) after harvest from E16.5 doubly transgenic Wnt1::FlpeER^T2; R26::FRAP embryos. Induction of FlpeER^T2-mediated recombination by TAM administration separates temporal cohorts of Wnt1 (rhombic lip)-derived hCPe. E7.5 and E8.5 TAM administration (∼E8 and ∼E9 recombination in the rhombic lip) resulted in no labeled E16.5 hCPe cells. (C) TAM at E10.5 (recombination in the rhombic lip at ∼E11) resulted in numerous labeled cells in the E16.5 hCPe. Insets reveal no PLAP activity when diluent alone was administered. rec, recombination time point; harv, harvest time point.