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Fig. 1. Roof plate epithelium in the hindbrain. (A) Cartoon of a dorsal view of the neural tube (E11.5); neuroepithelium of the rhombic lip is dark gray and hindbrain roof plate epithelium (hRPe) is light gray. The rhombic lip in rhombomere 1 (r1) is the cerebellar or upper rhombic lip (URL, white arrowhead); that within r2-r8 is the hindbrain or lower rhombic lip (LRL, black arrowhead). Broken line marked by `b' indicates the level of the idealized transverse section shown in B and the section plane in subsequent figures. Broken line marked by `d' indicates the axial level of idealized transverse section through the spinal neural tube depicted in D. (B) Vertical bracket demarcates the dorsoventral (DV) region of the rhombic lip (RL); horizontal brackets demarcate medially (med) versus laterally (lat) located hRPe (relevant to subsequent figures). Boxed area (c) is further schematized in C. (E) Schematic of cumulative and inducible (temporal) fate mapping. A broadly active promoter (BAP) drives expression of an indicator transgene containing either an FRT- or loxP-flanked stop cassette followed by a sequence encoding a reporter molecule that serves as a lineage tracer. Transcription of the reporter is prevented by the stop cassette (top); excision by Flpe or Cre (or following FlpeER^T2-mediated recombination in the presence of tamoxifen), permits reporter transcription (bottom). mb, midbrain; hb, hindbrain; ov, otic vesicle; 4v, fourth ventricle; vz, ventricular zone; mz, post-mitotic mantle zone; ect, epidermal ectoderm; mes, mesenchyme; RPe, roof plate epithelium.

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