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Fig. 7. The defects of hai1 mutants are phenocopied by overexpression
and rescued by knockdown of matriptase1a. (A) In situ
hybridization revealing matriptase1a (labelled mat1a)
expression in the epidermis, olfactory epithelium (olf), otic epithelium (ot),
gut (gt) and lateral line primordium (llp) at 24 hpf. (B)
Counter-staining with p63 antibody (brown), demonstrating expression in the
basal epidermal layer. (C,D) Lateral views of yolk sac extension
of heat-shock-treated embryos injected with
pTol2-hse-GTP/matriptase1a alone (C), displaying epidermal
dissociation, or co-injected with pTol2-hse-GTP/matriptase1a and
matriptase1a MO (D), displaying normal epidermal morphology; 24 hpf,
overlays of fluorescent and Nomarski images. Cells with transgene expression
are labeled by GFP. (E,F) Lateral Nomarski images of a 24 hpf
un-injected wild-type embryo (E) and matriptase1a morphant (F),
demonstrating that the loss of Matriptase1a does not affect epidermal
morphology. (G,H) Lateral Nomarski images of hai1a
mutants injected with either matriptase1a MO alone (G) or with
matriptase1a and hai1b MOs (H); both display normal
epidermal morphology. (I) Anti-p63 immunostaining of a 24 hpf
hai1a mutant co-injected with matriptase1a and
hai1b MOs. (J) Acridine orange (AO) staining alone (lower
panel) and super-imposed with a Nomarski image (upper panel) of the yolk
extension region of a 24 hpf hai1a mutant injected with
matriptase1a MO. (K) Stills of time-lapse Movie 8 in the
supplementary material (at the times indicated after 24 hpf), demonstrating
that matriptase1a MO injection restores the epithelial properties of
fluorescently labeled hai1a morphant basal keratinocytes.
(L,M) Lateral views of the trunk and tail of an un-injected
hai1a mutant (L) and of a hai1a mutant injected with
matriptase1a MO (M) after in situ hybridization for the leukocyte
marker lcp1 at 24 hpf.
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