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Fig. 3. The four mRNA targets of HOW are elevated in the mesoderm of
how germline clone embryos and exhibit direct binding to HOW.
Whole-mount wild-type (A-D) or how germline clone (E-H)
embryos hybridized with each of the four mRNAs identified in the microarray
screen: miple (A,E), lap (B,F), CG31638 (C,G) and
falten (D,H). Arrowheads in E-H indicate the specific increase in the
levels of the distinct mRNAs in the mesoderm of how germline clone
embryos. (I) HOW-RNA binding assay with in vitro-transcribed RNA of the
3' UTRs of miple, lap, CG31638, falten and punt.
HOWR185C is a form of HOW that is mutated in the KH RNA-binding
domain and served as a control for non-specific binding. The first two lanes
on the left represent total HOW protein levels. Each binding experiment shown
is representative of three repetitions.
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